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MOLECULAR CHARACTERIZATION OF CHROMOSOMAL AND EXTRA CHROMOSOMAL ELEMENTS RELATED TO ANTIBIOTICS RESISTANCE GENES IN SALMONELLA TYPHI
2021 - 06 - 19

Abstract

Salmonella typhi the causative agent of typhoid, the consider largest killer disease is becoming a major threat due to emerging antimicrobial resistance. Mechanisms underlying bacterial drug resistance involve developing and/or acquiring of genetic elements in the form of variety of genes, gene complexes or mutations.These genetic elements are either chromosomal and/or extrachromosomal in the form of plasmids. The present study was conducted to investigation of the presence antibiotic-resistance genes (cat, dfrA7, gyrA, gyrB, strA-strB, sulI, sulII, tetB) were done by using PCR technique and electrophoresis systems. One hundred percent of S.typhi isolates were positive with gyrA, while 25 (83%) of isolates carry gyrB gene, 19 (63.3%) of isolates carry gene cat, dfrA7, 12(40%) and 6 (20%) isolates were carry sulII and sulI, respectively. Thirty (100%) of the isolates gave a negative genes results of (strA-strB, tetB).

DOL: -9752366
ISBN: --


Tags
S. typhi, antibiotics resistance genes.
MOLECULAR CHARACTERIZATION OF CHROMOSOMAL AND EXTRA CHROMOSOMAL ELEMENTS RELATED TO ANTIBIOTICS RESISTANCE GENES IN SALMONELLA TYPHI

Abstract

Salmonella typhi the causative agent of typhoid, the consider largest killer disease is becoming a major threat due to emerging antimicrobial resistance. Mechanisms underlying bacterial drug resistance involve developing and/or acquiring of genetic elements in the form of variety of genes, gene complexes or mutations.These genetic elements are either chromosomal and/or extrachromosomal in the form of plasmids. The present study was conducted to investigation of the presence antibiotic-resistance genes (cat, dfrA7, gyrA, gyrB, strA-strB, sulI, sulII, tetB) were done by using PCR technique and electrophoresis systems. One hundred percent of S.typhi isolates were positive with gyrA, while 25 (83%) of isolates carry gyrB gene, 19 (63.3%) of isolates carry gene cat, dfrA7, 12(40%) and 6 (20%) isolates were carry sulII and sulI, respectively. Thirty (100%) of the isolates gave a negative genes results of (strA-strB, tetB).

DOL: -9752366
ISBN: --

Tags
S. typhi, antibiotics resistance genes.